Use of mPEGEnzyme Mixed Disulfides in Conjunction with Ion Exchange Chromatography

During the mid-1990s Looze and co-workers introduced the use of soluble mixed disulfides containing the usual 2-mercaptopyridine leaving group and derivatives of monomethoxypolyethylene glycol (mPEG; nominal molecular mass 5 kDa) (Figures 3D and 3E) for the isolation of thiol-containing enzymes by ion exchange chromatography. The chromato-graphic behaviour of the enzymes appears to be modified by the charge shielding effects of the PEG chain. This approach provides another means of separating components of mixtures of thiol-enzymes as an alternative to sequential elution covalent chromatography.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook


Post a comment