Type V Chiral Stationary Phases

Chiral stationary phases based on a protein are of special interest because of their unique properties of stereoselectivity and because they are suited for separating a wide range of enantiomeric mixtures. Protein-based stationary phases so far developed have included albumins such as bovine and human serum albumin, enzymes such as trypsin, a-chymotrypsin, lysozyme and pepsin, and glycoproteins such as a1-acid glycoprotein from human or bovine serum, cellobiohydrolase I from the fungus Trichoderma reesei, ovomucoid (in fact, ovoglycoprotein), avidin and ovotransferrin from egg whites, and flavoprotein (riboflavin-binding protein) from egg whites and yolks. The advantages of protein-based stationary phases generally include the use in reversed-phase mode, enantioselectivity for a wide range of compounds and direct analysis without derivatization. The disadvantages are low capacity, lack of column ruggedness and limited understanding of the chiral recognition mechanism. These stationary phases are mainly used for analytical purposes.

With regard to the chiral recognition mechanism, hydrophobic, electrostatic and hydrogen-bonding interactions take place between the chiral stationary phase and the solute.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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