Two Dimensional TLC

Another powerful technique for resolving the aflatoxins from interferences is two-dimensional TLC. In this technique two spots of aflatoxin standards and one spot of sample extract are spotted on the plate, as shown in Figure 2. The plate is first developed with ethyl ether-methanol-water (96:3 : 1 v/v) in the first direction. After development and air drying, the plate is redeveloped in the second direction with acetone-chloroform (1 : 9 v/v). After development and air drying the plate is examined under ultraviolet light at 365 nm for aflatoxin spots. A blue spot should appear at the intersection of imaginary lines from the two standard spots. The

Direction 2

0 1 ng

Standard spots

/ \

/ \

1 ng

/

\

0 20 |xL

0

Figure 2 Two-dimensional TLC plate for aflatoxin analysis.

Figure 2 Two-dimensional TLC plate for aflatoxin analysis.

two-dimensional technique works well for difficult materials such as eggs and spices.

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