Thin Layer CCD According to Albertsson

This CCD apparatus is designed to be used with aqueous two-phase systems which normally need a long time for settling. By using systems with low heights, only a few millimetres, the settling time will be acceptable (5-20 min). The operating unit (Figure 5) consists of two circular plates of poly-acrylic plastic, one on top of each other: the lower plate (stator) is fixed while the upper one (rotor) can be rotated stepwise. Both plates have 60 (or 120) radially oriented cavities which pair-wise form containers for the two-phase systems. The cavities of the stator contain the lower phases (normally 0.8 mL) while the upper phases (0.2-2 mL) are situated in the

Figure 5 Thin-layer CCD apparatus according to Albertsson. (A) The two discs of polyacrylic plastic contain matching cavities for the upper and lower phases and by rotation the upper plate can move all upper phases relative to the lower ones. See text for further details. (Adapted from Albertsson P-A (1986) Partition of Cell Particles and Macromolecules, 3rd edn, p. 126. New York: Wiley (interscience), by permission.) (B) A thin-layer CCD machine constructed by Albertsson.

Figure 5 Thin-layer CCD apparatus according to Albertsson. (A) The two discs of polyacrylic plastic contain matching cavities for the upper and lower phases and by rotation the upper plate can move all upper phases relative to the lower ones. See text for further details. (Adapted from Albertsson P-A (1986) Partition of Cell Particles and Macromolecules, 3rd edn, p. 126. New York: Wiley (interscience), by permission.) (B) A thin-layer CCD machine constructed by Albertsson.

cavities of the rotor and therefore can be transferred relative to the lower phases by rotating the upper plate. The two plates are placed on the shaking table of a machine which also contains a drive for the upper plate rotation. The phase systems are added to each container (chamber) via openings in the upper side of the rotor. These openings, during the run, are covered with a ring-formed lid. The shaking and settling periods and number of transfers are controlled by an automatic unit. After the CCD run is completed the two-phase systems can be collected by use of a fraction collector. This is a circular rack with the same number of test tubes (4 mL) as chambers. The rack is placed over the inlet holes and is inverted together with the plates which are then emptied.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook


Post a comment