The Pre Pyridyl Disulfide

The first example of covalent chromatography dates from 1963 when Eldjarn and Jellum reported the use of an organomercurial dextran based on Sephadex G-25 (Figure 1A) for the isolation of thiol-containing proteins which are released from the gel by treatment with low Mr mercaptans. Simpler and more effective products using a better support material (agarose) were developed subsequently, e.g. by Cuatrecasus

Figure 1 Some organomercurial gels. (A) Due to Eldjarn and Jellum, 1963. (B) Due to Cuatrecasus, 1970. (C) Due to Sluyter-man and Wijdenes, 1970.

(Figure 1B) and by Sluyterman and Wijdenes (Figure 1C) both in 1970. Some solid phase organomercurials are available commercially but as Lozinskii and Rogozhin pointed out in their review in 1980 (Table 1) relatively little interest was shown in the technique of covalent chromatography until the introduction of the version involving thiol-disulfide interchange using a solid phase 2-pyridyl disulfide gel in 1973 (Table 1). Problems with organomercurial gels include gradual loss of the metal with consequent contamination of the purified protein, lack of absolute specificity for thiol groups and lack of provision both for designed selectivity and of a means of spectral monitoring of occupancy of gel sites by the target protein.

Solar Panel Basics

Solar Panel Basics

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