The Different Parameters

As has been shown above, the speed of the eluent is a complex function of time and space and will not give the desired constant flow rate as in HPLC. Accordingly the plate efficiency is also a complex function of experimental conditions.

z and z0 have been previously defined and (z — z0)RF is the migration distance of the solute; a2 is the variance of the spot to which the contribution due to the starting spot width should be added.

We shall here treat the TLC plate as being equivalent to a normal chromatographic column bed: it is homogeneous, there is one dimensional flow (no vaporization or adsorption). This may seem oversimplified, but most conclusions apply in practice, giving some validity to the model.

We use the conventional Knox reduced plate height equation:

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment