The Chain Length Parameter

A general systematic approach to the purification of proteins via hydrophobic interactions was initiated by Shaltiel who introduced the principle of variation of the immobilized alkyl chain length in the form of the homologous series of hydrocarbon-coated agaroses (Seph-Cn, n = 1-10). The major conclusion of his experimental result was that an increase of the chain length by -CH2- units concomitantly increased the strength of protein binding from retardation to reversible binding up to very tight binding ('irreversible' binding). In addition to this variation in binding affinity with the chain length, the gels also changed their specificity towards the adsorbed protein. Thus it was suggested that the properties of hydrophobic agaroses for protein purification could be optimized by variation of the immobilized alkyl chain length.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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