Tetracyclines

Various tetracyclines are analysed with an octyl column using a mobile phase of methanol-acetonit-rile-0.01 mol L"1 aqueous oxalic acid solution (pH adjusted to 2.0 with 28% aqueous ammonia, 1 : 1.5 : 5) and detection at 360 nm.

Tetracycline (TC), chlortetracycline (CTC), doxycycline, minocycline, oxytetracycline (OTC), impurities of these tetracyclines including 4-epitet-racycline, anhydrotetracycline and 4-epianhydrotet-racycline (a nephrotoxic degradation product) are resolved with an ODS column using a gradient system (see Figure 7 for chemical structures). The mobile phase is an aqueous solution of 1mmolL"1 tetraammonium ethylenediamine tetraacetate and 50mmolL"1 diethanolamine (pH adjusted to 7.3 with 85% phosphoric acid) containing 2-10% iso-propanol. Tetracyclines are detected at 254 nm. Impurities of tetracycline are also analysed with an ODS column using a mobile phase consisting of meth-anol-acetonitrile-0.2 mol L"1 aqueous oxalic acid solution (pH adjusted to 2.0 with 28% aqueous ammonia, 1 : 1 : 3.5). Tetracycline and impurities are detected at 400 nm.

TC, CTC and OTC in plasma and urine are analysed with an ODS column using a mobile phase consisting of 0.01 mol L"1 phosphate buffer (pH 2.4)-acetonitrile (7 : 3 or 6 : 4). Tetracyclines are detected at 355 nm with a detection limit of 1 |g mL"1. Extraction of tetracyclines from biological fluids into

Solar Panel Basics

Solar Panel Basics

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