Temafloxacin enantiomers in biological fluids are extracted with methylene chloride and analysed by two types of HPLC method with derivatization. For the first method, temafloxacin enantiomers are reacted with S-( — )-N-1-(2-naphthylsulfonyl)-2-pyrrolidine carbonylchloride to form diastereomers, which are injected into a silica gel column. The mobile phase is hexane-methyl acetate-methanol-ammonia water (150 : 100 : 10 : 1) with UV detection at 280 nm. The detection limit is 5ngmL"1 for each diastereomer with a separation coefficient of 1.05.

For the second method, temafloxacin is reacted with acetic anhydride to form acetylated tema-floxacin followed by reaction with isobutylchlorofor-mate to form cabonylamidated derivatives. These double-derivatized temafloxacin enantiomers are analysed with an ovomucoid conjugated silica gel column using a mobile phase of 0.02 mol L"1 phosphate buffer (pH 7.0)-acetonitrile (92 : 8). The enan-tiomers are detected at 280 nm. The detection limit is 5ngmL"1 for each enantiomer with a separation coefficient of 1.50, indicating a better resolution by the second method.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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