Temafloxacin

Temafloxacin enantiomers in biological fluids are extracted with methylene chloride and analysed by two types of HPLC method with derivatization. For the first method, temafloxacin enantiomers are reacted with S-( — )-N-1-(2-naphthylsulfonyl)-2-pyrrolidine carbonylchloride to form diastereomers, which are injected into a silica gel column. The mobile phase is hexane-methyl acetate-methanol-ammonia water (150 : 100 : 10 : 1) with UV detection at 280 nm. The detection limit is 5ngmL"1 for each diastereomer with a separation coefficient of 1.05.

For the second method, temafloxacin is reacted with acetic anhydride to form acetylated tema-floxacin followed by reaction with isobutylchlorofor-mate to form cabonylamidated derivatives. These double-derivatized temafloxacin enantiomers are analysed with an ovomucoid conjugated silica gel column using a mobile phase of 0.02 mol L"1 phosphate buffer (pH 7.0)-acetonitrile (92 : 8). The enan-tiomers are detected at 280 nm. The detection limit is 5ngmL"1 for each enantiomer with a separation coefficient of 1.50, indicating a better resolution by the second method.

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Solar Panel Basics

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