Hydrodynamic chromatography (HDC) is one of the many techniques for particle size determination in the micron range. It has some similarities with size exclusion chromatography and field flow fractionation, but needs only one phase and one field. The main advantages are the separation of species according to size only, rapidity of measurement in the untreated medium and ease of operation of equipment. Variation in operating parameters allows a considerable molecules, which can be extremely useful when dealing with completely unknown biopolymers. The errors involved are significantly greater for the materials of smaller relative molecular mass because the response (the amount of light scattered) is much less, and the output signal is much closer to the noise level of the sensing system. Further discussion of these types of detector are furnished in references provided in the Further Reading section.

See also: N/Chromatography: Liquid:Detectors: Evaporative Light Scattering. Chromatography: Protein Separation. Appendix 1/Essential Guides for Isolation/ Purification of Drug Metabolites. Essential Guides for Isolation/Purification of Enzymes and Proteins.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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