Summary

Detection and quantitation of bile acids are important in health and disease. Bile acid synthetic defects may arise from both defective cholesterol synthesis and metabolism; bile acid therapy may correct some of these abnormalities. GC has proved to be of vital importance in determination of bile acids in biological fluids. However, a complete GC analysis generally takes a long time. By appropriate column and derivatization selection, analysis time can be significantly reduced, but sample preparation for GC analysis is usually time-consuming. Combination of GC and mass spectrometry greatly aids in the characterization of unknown bile acids. In attempts to get additional information, Child et al. have developed a method to quantitate sterols and fatty acids together with bile acids in stool samples. Advantage is taken of the greatly increased retention times of the n-butyl ester-acetates of bile acids, so that sterol acetates are eluted earlier than all bile acids present and a group separation of faecal fatty acids, sterols and bile acids is achieved. In a further advancement of the method, Batta et al. have prepared n-butyl esters of bile acids directly in faecal samples, followed by trimethylsilyl ether formation. They showed that

K. Saar, Aventis Research & Technology, Frankfurt, Germany

S. Muillner, Enzyme Technology, Dusseldorf, Germany Copyright © 2000 Academic Press

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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