Stationary Phases for IPTLC

A range of stationary phases have been used for IP-TLC including silica gel, paraffin-coated silica gel

Figure 3 Lack of effect of pH on the values of some dihydroxybenzoic acids on IP-TLC using tetrabutylammonium reagents on (A) paraffin-coated silica gel TLC plates and (B) C18-bonded TLC plates. ■, gentisic acid; 2,5-dihydroxybenzoic acid; O, 2,6-dihydroxybenzoic acid; ▲, salicylic acid.

and a range from C2, C8, cyanopropyl, aminopropyl, diphenyl to C18-bonded layers. Chromatography of polar ionic analytes can be obtained with a range of IP reagents on silica gel, paraffin-coated silica gel, alkyl-bonded (C2 to C18) and diphenyl-bonded layers. However, it is our experience that aminopropyl layers give poor results with IP reagents, and that spot shapes on cyanopropyl layers are poor. It should be noted that these comments are based on limited studies and relatively little work has been done to investigate the use of some of these layers (e.g. cyanopropyl, aminopropyl and diphenyl). It should also be noted that not all reagents can be used with all phases. For example, tetrabutylammonium bromide can be employed with alkyl-bonded stationary phases or paraffin-coated silica gel, but is ineffective on bare silica. In contrast, cetrimide works well on both bonded stationary phases and silica gel.

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