Separation System for ITP Analyser

A typical diagram of ITP analyser is shown in Figure 3 for unidirectional ITP. A separation tube connecting two electrodes is made of polytetra-fluoroethylene (PTFE) or fused silica, whose inner diameter is 0.2-0.5 mm. A PTFE tube as large as 1 mm inner diameter is frequently used before detection as a pre-column to increase column hold up or electric charge for better resolution. An additional leading electrode chamber can be used to apply high current during pre-separation to reduce analysis time. The chamber is connected appropriately to the separation tube before the detector. The leading and the terminating electrode chamber are typically 20 mL in volume.

The separation tube is also filled with a leading electrolyte and a terminating electrolyte. The boundary is formed at a sample injection port and a sample solution is introduced there typically by using a microsyringe. Sample components are separated during migration and their zones are detected using appropriate detectors.

Solar Panel Basics

Solar Panel Basics

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