Separation Strategy and Typical Applications

Operational electrolyte conditions should be optimized to obtain the best quality of separation by changing electrolyte parameters so that the difference of the effective mobilities of the target components should be as large as possible. Strictly speaking, the separability depends on the mobility difference in the mixed zone (see Figure 1). However, to a first approximation, the mobility differences at the steady state or the difference of the RE values may be used for the criterion for optimization of separations.

For separation optimization in electrophoresis, a theoretical approach is sometimes very useful. In isotachophoresis especially, the pH and the ionic strength of the separated zones are different to one other. The optimum electrolyte system can be determined by iterative computer calculations where the effective mobilities of the species being separated at the steady state are calculated using their physicochemical constants, and the differences of the effective mobilities compared. This method may be called computer-aided separation optimization, which enables the determination of the electrolyte conditions such as the pH without time-consuming 'trial-and-error' experiments. Some examples of simulation are also included in this section.

Solar Panel Basics

Solar Panel Basics

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