Scan Functions in Tandem Mass Spectrometry

Although there are several scan functions that can be performed by tandem instruments, the most commonly used function in TLC/HPTLC applications is the product ion scan. The analytical characteristics of TLC/HPTLC-MS-MS product ion analysis contrast it with analysis by chromatography-chromatogra-phy-mass spectrometer.

Even so, just as with conventional MS, tandem mass spectrometry has its limitations. MS-MS cannot easily differentiate between isomeric and isobaric species. Isomers tend to produce similar product ion spectra and, since they cannot be separated by mass, two isomers transmitted into the gas cell simultaneously will give a product ion spectrum that is representative of the sum of the individual compounds.

Similarly, if isobaric components of the same elemental composition but of different structure are focused by the first analyser, transmitted into the gas cell and dissociated by collisions, the product ion spectrum obtained from the second analyser will be a compilation of product ions from the two components. It can be seen, therefore, that some form of chromatographic separation such as TLC/HPTLC prior to ionization can enhance the separative and characterization powers of the tandem mass spectrometer. Conversely, the use of MS-MS can greatly enhance the identification and characterization of isomeric and isobaric species separated by planar chromatography.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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