Sample Application

As the first step of planar chromatography, sample application largely determines the overall quality of the separation. All analytes and standards are chromatographed and compared with each other on the same plate, generally by means of migration distance or RF value. Therefore, exact positioning of the sample during application is crucial for both qualitative and quantitative TLC. Quantitative work also requires exact reproducibility of applied volumes. Furthermore, in order to utilize fully the separation power of the layer, it is important to restrict the dimension of the sample origin in the direction of chromatography. The advantage of instrumentation is mainly derived from a much higher reproducibility of all these parameters.

Generally, two principal sample application techniques are distinguished: application of spots and application of bands. The selection of a sample

Solar Panel Basics

Solar Panel Basics

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