Rotation Planar Chromatography

The term 'rotation planar chromatography' (RPC) -irrespective of the type and quality of the stationary phase - embraces analytical, offline micropreparative and online preparative forced-flow planar chromato-graphic techniques in which the mobile phase migrates mainly with the aid of centrifugal force, but also by capillary action. The centrifugal force drives the mobile phase through the sorbent from the centre to the periphery of the plate. The mobile phase velocity may be varied by adjustment of the speed of rotation.

The different RPC techniques can be classified as normal chamber RPC (N-RPC), micro chamber RPC (M-RPC), ultramicro chamber RPC (U-RPC) and column RPC (C-RPC); the difference lies in the size of the vapour space, an essential criterion in RPC. For analytical separations many samples can be applied. For micropreparative and preparative purposes only one sample is applied as a circle near the centre of the rotating stationary phase. The separations can be performed either in the offline or online mode. In the latter, the separated compounds are eluted from


the stationary phase by the centrifugal force and collected in a fraction collector (Figure 10). All methods can be used for online preparative separations; M-RPC and U-RPC can also be used for analytical and offline micropreparative separations.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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