Pulsed Field for DNA

Agarose gel electrophoresis is the method of choice to resolve DNA restriction fragments provided the fragments are between 1000 and 23 000 bp in size. For larger fragments, Schwartz and Cantor developed the technique of pulsed field gel electrophoresis (PFG) in 1984. In PFG DNA fragments greater than 23 kbp are forced to change their structure during elec-trophoresis by pulsing the applied field so causing the molecules to relax and expand regularly and thereby interact with the gel pores. DNA molecules up to the size of chromosomes can be electrophoresed in this manner, but the separation can take many hours or even days.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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