Proteins Detection of

97 W

97 W

Figure 1 SDS-PAGE separation of human heart proteins (lanes b-g). Lane (a) contains the molecular weight marker proteins and the scale at the left indicates protein size in kDa. The gel has been stained with Coomassie Brilliant Blue R-250. The sample protein loadings were (b) 1 ^g, (c) 5 ^g, (d) 10 ^g, (e) 25 ^g, (f) 50 ^g, (g) 100 ^g.

Figure 1 SDS-PAGE separation of human heart proteins (lanes b-g). Lane (a) contains the molecular weight marker proteins and the scale at the left indicates protein size in kDa. The gel has been stained with Coomassie Brilliant Blue R-250. The sample protein loadings were (b) 1 ^g, (c) 5 ^g, (d) 10 ^g, (e) 25 ^g, (f) 50 ^g, (g) 100 ^g.

More sensitive staining (down to 10 ng protein per band) can be achieved using the dimethylated form of the dye, CBB G-250, as a 0.1% w/v colloidal dispersion in 2% w/v phosphoric acid, 10% w/v ammonium sulfate, and 20% v/v methanol. An additional advantage of this method is that the colloidal dye only binds to the separated proteins as it is unable to penetrate the gel matrix. This means that no de-staining step is required and the intensity of staining can be controlled by visual inspection during the staining process. Related dyes such as Acid Violet 17, Serva Violet 49 and Fast Green FCF also form colloids in strongly acidic solutions and stain proteins in gels with low background.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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