Protein Identification

Several approaches have been used to identify proteins after 2D-PAGE. Co-migration with purified known proteins and Western blotting were employed by the pioneers of the 2D field. The use of specific antibodies and the recent developments of antigen-antibody interactions with enhanced chemi-luminescence allow detection and identification of traces of proteins. However, monoclonal antibodies may not detect denatured polypeptides. Nowadays, proteins are identified by microsequencing, amino

Figure 3 Genetic heterogeneity of plasma proteins. Close-up of the human plasma 2D map corresponding to the haptoglobin P chain (pH 3.5-10 carrier ampholyte gradient). (A) L form (homozygous); (B) L-R forms (heterozygous); (C) R form (homozygous). A reference spot is shown by the arrowhead. This genetic polymorphism is less detectable with immobilized pH gradients. Note the microheterogeneity of haptoglobin P chain spots, which present both charge and size heterogeneities.

Figure 3 Genetic heterogeneity of plasma proteins. Close-up of the human plasma 2D map corresponding to the haptoglobin P chain (pH 3.5-10 carrier ampholyte gradient). (A) L form (homozygous); (B) L-R forms (heterozygous); (C) R form (homozygous). A reference spot is shown by the arrowhead. This genetic polymorphism is less detectable with immobilized pH gradients. Note the microheterogeneity of haptoglobin P chain spots, which present both charge and size heterogeneities.

acid analysis, peptide mass fingerprinting and/or mass spectrometry. The development of automated, high throughput technologies for the rapid identification of proteins is in progress. Automation already exists in several stages of the protein identification process. This includes automated acquisition of matrix-assisted laser desorption ionization-time-of-flight mass spectra and peptide mass fingerprinting. Bioinformation allows identification of proteins by mixing several databases (a classified index can be found at the following address: http://expasy. hcuge.ch/alinks.html # Proteins).

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook


Post a comment