Principles of SRPC

For difficult separation problems a special combination of circular and anticircular development can be performed with the sequential rotation planar chromatography (S-RPC). The mobile phase can be introduced onto the plate at any desired place and, time. In S-RPC the solvent application system - a sequential solvent delivery device - works by centrifugal force (circular mode) and with the aid of capillary action against the reduced centrifugal force (anticircular mode). Generally the circular mode is used for the separation, the anticircular mode for pushing the

Figure 11 Schematic diagram of preparative M-RPC. 1 = lower part of the stationary chamber, 2 = collector, 3 = motor shaft with the rotating disc, 4 = glass rotor, 5 = stationary phase, 6 = quartz glass cover plate, 7 = mobile phase inlet, 8 = eluent outlet.
Figure 12 Schematic diagram of preparative C-RPC. 1 = lower part of the stationary chamber, 2 = collector, 3 = motor shaft with the rotating disc, 4 = rotating planar column, 5 = stationary phase, 6 = quartz glass cover plate, 7 = mobile phase inlet, 8 = eluent outlet.

substance zones back to the centre with a strong solvent (e.g. ethanol). After drying the plate with nitrogen at a high rotation speed, the next development with another suitable mobile phase can be started. By combination of the two modes of operation the separation pathway in S-RPC becomes theoretically unlimited.

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