Preparative RPC Separations

Because all preparative RPC separations are performed online and no removal of the zones by scratching is necessary, the separation efficiency for the last eluting compounds even increases during the run.

Because M-RPC and U-RPC can be used not only for online preparative separations, but also for analytical purposes, direct scale-up is possible for both analytical methods. From TLC separations using un-saturated or saturated chromatographic tanks, the mobile phase can be transferred via analytical U-RPC and M-RPC to preparative U-RPC and M-RPC, respectively, if the solvent strength and selectivity are kept constant. For scale-up the sample may be applied as a circle to a 20 cm x 20 cm analytical TLC plate and the amount of sample can be increased stepwise in subsequent separations. The adverse effects of different particle sizes and separation distances in the analytical and preparative methods almost cancel each other, so only layer thickness has to be considered in the scale up procedure. The mobile phase flow rate must be adapted to preparative separation, so that the migration of the a front is as fast as in the analytical separation.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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