Preparation of the Sample Solution

In CCC, the sample solution is usually prepared by dissolving the sample in the solvents used for the separation. If the amount of sample is small, it may be dissolved in the stationary phase. However, if the sample mixture contains multiple components with a wide range of polarity, it should be dissolved in both solvent phases. In this way, the volume of the sample solution is minimized and also the two-phase formation in the sample solution is ensured. Occasionally, a single phase is formed after dissolving a large amount of the sample, which would result in a detrimental loss of the stationary phase from the column. If this occurs, the sample solution should be diluted with the solvent until two phases are formed. Although CCC permits the loading of the sample solution containing undissolved particulates, the best results are obtained by filtering the sample solution before introduction into the column.

As in other forms of LC, the amount of sample and sample solution affects the separation. Usually, a typical semipreparative column with about 300 mL capacity will separate up to a few hundred milligrams of sample dissolved in 10 mL of the solvent mixture without significantly affecting the partition efficiency.

Solar Panel Basics

Solar Panel Basics

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