PHZonerefining CCC for Chiral Separation

pH-Zone-refining CCC is a powerful preparative technique that yields a succession of highly concentrated rectangular solute peaks with minimum overlap where impurities are concentrated at the peak boundaries. This technique has been applied to the resolution of DNB-amino acid racemates using a binary two-phase solvent system where the retainer acid and chiral selector were added to the organic stationary phase and the eluent base to the aqueous mobile phase. Figure 4 shows a typical chromato-gram of a DNB-leucine racemate obtained by pH-zone-refining CCC. The pH of the fraction (dotted line) reveals that the peak is evenly divided into two pH zones with a sharp transition. Compared with the standard CCC technique, the pH-zone-refining CCC technique allows separation of large amounts of analyte in a shorter elution time.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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