Overpressured Layer Chromatography

In addition to capillary action, the force driving solvent migration in OPLC is the external pressure. Depending on the desired mobile-phase velocity, operating pressures up to 50 bar can currently be used. In OPLC (Figure 2) the vapour phase is completely eliminated; the chromatographic plate is covered with an elastic membrane under external pressure, thus the separation can be performed under controlled conditions. The absence of any vapour space must

Figure 2 Schematic diagram of online OPLC. 1, Support block; 2, chromatoplate; 3, support plate; 4, spring; 5, casette system for fixing the chromatoplate between two Teflon layers; 6, Teflon layer; 7, Mobile phase inlet; 8, mobile phase outlet; 9, hydraulic system.

be considered in the optimization of the solvent system, especially in connection with the disturbing zone and multifront effect, which are specific features of the absence of a vapour phase (see section entitled 'Elimination of Typical Problems With Use of OPLC').

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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