Optimization of Hydrophobic Chromatographic Systems

Twenty years after the introduction of hydrophobic interaction chromatography, the method has not gained the same foothold in the methodological repertoire of protein chemistry as has affinity chromatography. Although a large number of proteins have been successfully purified by this method a recent paper by Oscarsson et al. comes to the conclusion that certain 'classical' commercial hydro-phobic adsorbents are inadequate for ideal downstream processing because of their high hydrophobic-ity. The criticism of these authors is essentially correct. The major problem encountered on such hydro-phobic gels is that proteins can be very effectively adsorbed but elution in the native state is often impossible. Although a similar problem can be encountered in affinity chromatography, it appears to be the major handicap in hydrophobic interaction chromatography and must be taken into account in any general optimization procedure.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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