Oligonucleotidebased Ligands

Oligonucleotide (DNA or RNA)-based combinatorial chemistry has been exploited in a technology called SELEX (systemic evolution of ligand by exponential enrichment) to identify and isolate high affinity ligands directed for proteins, nucleic acids and low molecular weight targets such as peptides. The Kd values for such types of ligands are between 10 and 10_12molL_1 for proteins and between 10"6 and 10"9 mol L"1 for low molecular weight targets. The methodology involves random condensation of a mixture of activated monomers for generation of a combinatorial array of nucleic acid sequences that are assessed for binding towards any target (protein, nucleic acid, peptide or low molecular weight compound). The sequences are amplified by polymerase chain reaction, and the ones that show positive activity are selected and reamplified. The numbers of sequences that can be generated by commercially available synthesizers have been reported to be 1014-1015 in literature. The multiple rounds of selection exponentially enrich the group of oligonucleotide ligands with high affinity for the target.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment