OKeefe Partitioning

The multiplicative partitioning can be carried in such a way that a new portion of the solute mixture to be separated is added after each transfer step. This method of carrying out the partitioning is called O'Keefe partitioning. Several two-phase systems are arranged in a row and a solute sample is included in the centre system. After equilibration and settling all the upper phases are moved one step to the right and the overflowing upper phase is collected. Then all the lower phases are moved one step to the left and the one to the extreme left is collected. The remaining set is completed with one fresh lower phase (to the right) ane one fresh upper phase (to the left) to restore the original number of systems. After addition of a new portion of solute to the centre system the next cycle takes place. Substances with G > 1 will be recovered in the upper phases collected to the right and substances with G < 1 in the lower phases removed to the left. This process is used when large amounts of substances are to be separated. The number of systems is usually small, e.g. 7, 9 or 11.

A similar process, called Watanabe-Morikawa partitioning, differs in that the ongoing addition of solute mixture is done in the first system of the row. This is useful when only components with high or low G values are to be isolated.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment