Ofloxacin

Serum and urine samples are diluted with 0.1 mol L"1 phosphate buffer (pH 7.0) and extracted with dichloromethane. Ofloxacin enantiomers in the extract are reacted with l-leucinamide to form dias-tereomers. The diastereomers are extracted with 1 molL"1 HCl, and injected into an ODS column. The mobile phase is 0.2 mol L"1 phosphoric acid (with the pH adjusted to 1.85 with tetraethylam-monium hydroxide)-acetonitrile (4:1) with fluorescence detection at 298 nm excitation and 458 nm emission. The derivative of the S-( — )-enantiomer elutes prior to that of the R-( + )-enantiomer with baseline separation. Detection limits are 3 and 80 ng mL"1 for plasma and urine, respectively.

Ofloxacin enantiomers are also analysed using a chiral stationary phase (bovine serum albumin

Figure 6 Chemical structures of lomefloxacin (19), ofloxacin (20) and temafloxacin (21).

Figure 6 Chemical structures of lomefloxacin (19), ofloxacin (20) and temafloxacin (21).

covalently bonded to silica) without derivatization. Mobile phase is 0.2 mol L"1 phosphate buffer (pH 8.0)-2-propanol (97:3). Enantiomers are detected fluorometrically at 298 nm excitation, 458 nm emission. Resolution and sensitivity are poorer than those for the above derivatization method.

For clinical use, ofloxacin has been changed to levofloxacin which is the pharmacologically active S-( — )-enantiomer.

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