Off ine Solid Phase Sample Application

Preparative solid-phase sample application is especially useful whenever a large amount of sample must be applied and/or the sample is soluble in nonvolatile solvent only. The sample must be dissolved in a suitable solvent and mixed with approximately 5-10 times its weight of deactivated sorbent. The sorbent with the adsorbed sample is dried and then introduced into a layer which has to be prepared to accept it. A 180 mm x 5 mm channel with a U-shaped profile can be scratched from the stationary phase. After removal of the sorbent from the channel, the prepared sorbent with the adsorbed sample is placed in the channel and pressure is applied to ensure optimum contact between the stationary phase of the chromatographic plate and the applied sample. Offline solid phase sample application is useful not only for conventional PPC but also for linear OPLC and C-RPC separations. In OPLC the prepared plate is placed horizontally in the OPLC chamber and the separation can be started with a relatively high inlet pressure. Because of the forced flow, any possible lack of suitable contact has no effect on the efficiency of the separation. With C-RPC the column must first be filled with stationary phase, then with the solid sample. Because of the centrifugal force no lack of suitable contact can occur.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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