Modes of Liquid Chromatography

Analytical LC is always carried out by elution chromatography where the mobile phase is passed continuously through the column and the sample is fed (or injected) into the system as a finite plug. If the conditions for the analysis are optimized, the sample components can be completely separated from each other. Placing a detector at the end of the column, which responds to some property of the sample, produces a trace (the chromatogram) which is a plot of detector response against time.

If the stationary phase is more polar than the mobile phase, the term normal-phase chromatography is used, and if the stationary phase is less polar than the mobile phase the term reversed-phase chromatography is used.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook


Post a comment