Mobile Phase Reservoir

The mobile-phase reservoir can be any clean, inert container. It usually contains from 0.5 to 2 L of solvent, and it should have a cap that allows for a tubing inlet line, which feeds mobile phase to the solvent-delivery system. The cap also serves to keep out dust, reduce solvent evaporation, allow for press-urization of the bottle, offer ports for additional inlet lines, and sparging (i.e. dispersing He or Ar into the

Figure 1 Generic HPLC system.

mobile phase to remove dissolved air). All mobile phases/solvents should be freshly filtered and preferably degassed. Online degassers, which are primarily used to remove small gas bubbles and reduce the amount of dissolved air, are now popular additions to many HPLC systems, and they eliminate the need to degas mobile phase offline. An additional filter is often placed at the end of the mobile-phase inlet line to remove any precipitants that may form in the mobile phase during its use. Sparging control and the ability to blanket the solvents with inert gases is highly recommended to eliminate carbonate formation in alkaline solvents and to maintain extremely low levels of dissolved oxygen, when performing electrochemical or fluorescence detection.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook


Post a comment