Mobile Phase Effects

A chromatogram of the Mesuximid enantiomer separation that clearly illustrates the separation power of this type of materials is depicted in Figure 1.

For Mesuximid, using «-hexane-2 propanol as the mobile phase, the investigation of the effect of the polar modifier concentration on the retention factor demonstrate that, below a certain polar modifier concentration, the retention factor strongly increases. This can be an indication that competition between the polar modifier and the solute for hydrogen-binding sites on the stationary phase might be the determining factor in the retention process.

Although the retention factor is strongly influenced by the polar modifier concentration, the effect on the

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Time (min)

Figure 1 Benzoyl cellulose beads in the pure polymeric form: effect of polar modifier concentration on the retention factor. Column: 125 x 4 mm i.d. filled with 10 |im p-methylbenzoyl cellulose beads. Mobile phase: n-hexane-2-propanol in different ratios. Flow rate: 1 mLmin"1. Sample: Mesuximid.

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Figure 1 Benzoyl cellulose beads in the pure polymeric form: effect of polar modifier concentration on the retention factor. Column: 125 x 4 mm i.d. filled with 10 |im p-methylbenzoyl cellulose beads. Mobile phase: n-hexane-2-propanol in different ratios. Flow rate: 1 mLmin"1. Sample: Mesuximid.

selectivity factor in the studied range of polar modifier content remains rather small.

The benzoyl cellulose derivatives in the pure polymeric form allow the separation of a broad variety of compounds. In many cases, results are obtained which are comparable with the values measured on the corresponding physically coated phases. Furthermore, these materials are very useful as stationary phases for preparative chromatographic applications.

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Solar Panel Basics

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