Micropreparative RPC Separations

A mixture of components (5-15 mg) can be applied in the form of a ring near the centre of a single analytical HPTLC plate for isolation of relatively pure compounds by use of U-RPC or M-RPC. The operating process is similar to that used for analytical separations, with the difference that only one sample is applied. Continuous development is possible if a ring of radius 9.8 cm is scraped from the stationary phase, to ensure the regular migration of the mobile phase after it has reached the outskirts of the plate. When the first compounds of interest reach this ring, the separation must be stopped, and either with a stationary rotor or at a rotation speed of 100 rpm the separated components can be scraped out and then the remaining substances can be eluted by the usual procedures, similar to preparative TLC. The separation of ultraviolet (UV)-active compounds can be monitored with a UV lamp during the separation.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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