Metal Chelate Stains

This recently developed group of stains have been developed specifically for compatibility with characterization methods such as mass spectrometry as they do not use reagents such as glutaraldehyde or formaldehyde which reduce their efficacy. Although a stain of this type, using the pink bathophenanthroline dis-ulfonate/ferrous complex, was described over twenty

Figure 3 SDS-PAGE separation of human heart proteins (lanes b-g). Lane (a) contains the molecular weight marker proteins and the scale at the left indicates protein size in kDa. The gel has been stained with SYPRO red. The sample protein loadings were (b) 1 ^g, (c) 5 ^g, (d) 10 ^g, (e) 25 ^g, (f) 50 ^g,

Figure 3 SDS-PAGE separation of human heart proteins (lanes b-g). Lane (a) contains the molecular weight marker proteins and the scale at the left indicates protein size in kDa. The gel has been stained with SYPRO red. The sample protein loadings were (b) 1 ^g, (c) 5 ^g, (d) 10 ^g, (e) 25 ^g, (f) 50 ^g,

years ago, its insensitivity (600 ng protein) ensured its lack of acceptance. Sensitivity can be increased by introducing 59Fe into the complex, but only to a level equivalent of colloidal Coomassie Blue staining.

The poor performance of these dyes resulted in a recent investigation of luminescent metal chelate stains. Such stains utilizing metal chelates complexed to certain transition metal ions (e.g. europium, terbium, and ruthenium) offer much greater sensitivity compared to the previous colorimetric methods. Of particular interest is SYPRO Ruby, a proprietary ruthenium-based metal chelate stain (Molecular Probes, Eugene, Oregon, USA). This allows one-step, low background staining of proteins in polyacrylamide gels without resorting to lengthy destaining procedures. The linear dynamic range of this dye extends over three orders of magnitude, thus surpassing silver and Coomassie stains in performance. Its sensitivity is claimed to be up to thirty times more sensitive than silver staining. Moreover, staining times (unlike silver protocols) are not critical and staining can be carried out overnight without overdevelopment.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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