Membrane Affinity Filtration

Chemical attachment of the affinity ligand to a filtration membrane provides the principle of the membrane affinity filtration technique. The role of the filter component can be taken by a hollow-fibre microporous membrane where the ligand is chemically attached. The feed stream moves along the interior of the fibre whose pores are large enough (0.5-1.0 |im) to permit convective flow through its body. While binding of the targeted protein to the immobilized ligand occurs without diffusion limitations, unbound substances pass through the fibre body to its outer surface. The main advantage offered by this technique, which is important to industrial-scale purification, is that there is no need for solids removal from the feed stream after cell disintegration. In fact, liquid-solid separation and affinity purification are combined and performed simultaneously when using this technique. Affinity membranes permit rapid processing of large volumes, sometimes even immediately after cell harvesting or disintegration, thus eliminating concentration and partial purification stages. Membrane systems other than hollow fibres have been developed, for example membrane discs and modules of flat membrane sheets bearing immobilized dyes. Such materials are effective tools in protein downstream processing.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook


Post a comment