Liquid Chromatography

J. Haginaka, Mukogawa Women's University,

Nishinomiya, Japan

Copyright © 2000 Academic Press

A pair of enantiomers only differ in their optical rotation, but their physical properties such as melting point, boiling point, refractive index and solubility are identical. On the other hand, the physical and chemical properties of a pair of diastereomers are different. For the separation of enantiomers by liquid chromatography, it is essential to form a dia-stereomeric complex in the mobile phase or in the stationary phase or to convert to diastereomers. The former is called the direct method, and is based on the addition of chiral additives to the eluent or the use of chiral stationary phases, resulting in the formation of a diastereomeric complex in the mobile or stationary phase. The second indirect method is based on the reaction of the enantiomers with a homochiral reagent, resulting in the formation of a pair of dia-stereomers. This article deals with both the indirect and direct methods using liquid chromatography.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment