Liquid Chromatographic Methods

With enzyme-based assays or other analytical methods like radioimmunoassays either total bile acid concentrations or single bile acids can be detected. Gas-liquid chromatography is a commonly used method for bile acid analysis, however, it does not allow the detection of conjugated bile acids. Therefore, in order to achieve relevant diagnostic data with regard to changes in the bile acid pattern, other specific separation techniques have to be employed.

In early studies on liquid chromatography of bile acids, silica gel was a commonly used stationary phase. Other materials such as aluminium oxide or neutral polymers were also used for separations under gravity pressure. Various solvent systems, e.g. mixtures of light petroleum, butanol, glacial acetic acid and other organic solvents have been described, mostly in combination with thin-layer chromatogra-phy (TLC) as a detection method. With the systems described, the separation of different groups into con-jugated/nonconjugated bile acids is possible, however poor sensitivity and insufficient selectivity are major drawbacks and make them unsuitable for routine diagnostics.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment