Large and/or polar compounds have a limited solubility in pure carbon dioxide. As has been already mentioned, in this case it is then necessary to add a modifier to the supercritical eluent to enhance the solubilities. The number of applications amenable to Prep-SFC is greatly increased by the use of modifiers. However, some classes of compounds are still insoluble in carbon dioxide and modifier. An example is that some peptides can be dissolved and processed by SFC, but proteins are outside of the range of Prep-SFC.

Another limitation of Prep-SFC is economic. Investment costs for Prep-SFC are much higher than for Prep-HPLC. Savings on solvent consumption will counterbalance the difference in investment cost only for large- or very large-scale applications. For small-or medium-size applications, where both techniques are applicable, HPLC will often be more economical than SFC.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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