Thin-layer chromatography (TLC) is a simple technique, many runs can be performed at the same time on a single plate, no detection problems occur provided a suitable reagent is used and moreover all solutes are detected. The main drawback is that a good detecting device designed for quantitative work is as expensive as a complete HPLC apparatus.

The observed decline of TLC is mainly due to the belief that efficiency is poor. To perpetuate that idea,

Pre-derivatization of sample on the TLC plate is proving to be a technique that often results in excellent resolution of analytes of interest. It is to be expected that further protocols will be developed in this area on the basis of reaction sequences that may already be available.

Tables showing the detection of compounds on TLC plates following chromatographic development can be found in appendix 17 on page 4801.

See Colour Plate 33.

See also: II/Chromatography: Liquid: Derivatization. Chromatography: Thin-Layer (Planar): Densitometry and Image Analysis; Instrumentation; Mass Spectrometry. III/Thin-Layer Chromatography-Vibration Spectro-scopy. Appendix 2/Thin-Layer (Planar) Chromatography: Detection.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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