Introduction to Applications

Dyes were originally introduced as cheap alternatives to costly nucleotide ligands for enzyme purification. Given the advantages of dyes over biological ligands, their broad protein-binding spectrum, and also the possibility of improving their selectivity for targeted proteins via molecular modelling, it is no surprise that these colourful tools are finding increasing application in various affinity-based biosepartion techniques. The literature abounds with examples where dye ligands have been employed to purify individual proteins and enzymes: albumin, antibodies, blood-clotting factors, plasminogen activator, proteolytic activities, growth factors, interferons, cellulolytic and lipolytic enzymes, collagenases, restriction endonucleases, and numerous nucleotide-binding proteins and enzymes, to name some examples. It is no exaggeration to claim that for almost every protein purification problem, a dye ligand can be found to help towards its solution.

See also: I/Affinity Separation. II/Affinity Separation:

Affinity Membranes; Affinity Partitioning in Aqueous Two-Phase Systems; Rational Design, Synthesis and Evaluation: Affinity Ligands. Membrane Separations: Membrane Bioseparations; Ultrafiltration. III/Enzymes: Liquid Chromatography. Proteins: Capillary Electrophoresis; Centrifugation; Electrophoresis. Pressurised Fluid Extraction: Non-Environmental Applications. Appendix 1/Essential Guides for Isolation/Purification of Enzymes and Proteins; Appendix 2/Essential Guides to Method Development in Affinity Chromatography.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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