According to J.N. Israelachvili (1985), hydrophobic interactions constitute 'the unusually strong attraction between nonpolar molecules and surfaces in water'. For two contacting methane molecules, the attraction energy is about sixfold higher in water than the van der Waals interaction energy in a vacuum. This energy, which has been estimated to be about — 8.5 kJ mol"1 for two methane molecules is due to the extrusion of ordered water on two adjacent hy-drophobic surfaces into less-ordered bulk water with a concomitant increase in entropy. This entropy-driven attraction between nonpolar groups in water is the basis for hydrophobic interaction chromatogra-phy.

The chromatographic separation of proteins depends on the differential accumulation of molecules at certain sites within a chromatographic system. Two principal types of chromatographic systems employing hydrophobic media have been described: (a) reversed-phase and (b) hydrophobic interaction chromatography. The principle of reversed-phase chromatography is based on a hydrophobic, e.g., silica, support of very high hydrophobicity which is capable of retaining nonpolar liquid phases (stationary liquid phase) when applied as the less polar phase in a solvent system. In this classical system the solutes are absorbed and separated (partitioned) in the apolar stationary liquid phase (i.e., a three-dimensional system) and not on the solid phase. In hydrophobic interaction chromatography the solutes (proteins) are adsorbed and separated on the apolar stationary solid phase (i.e., a two-dimensional system) carrying

Lowe CR, Burton S, Pearson J, Clonis YD and Stead CV (1986) The design and applications of biomimetic dyes in biotechnology. Journal of Chromatography 376: 121-130.

Lowe CR, Burton SJ, Burton NP, Alderton WK, Pitts JM and Thomas JA (1992) Designer dyes: 'biomimetic' ligands for the purification of pharmaceutical proteins by affinity chromatography. Trends in Biotechnology 10: 442-448.

immobilized hydrophobic groups. Because of the very different scopes and methodological details, re-versed-phase chromatography will not be treated here. The same holds for other forms of liquid-liquid partition chromatography. A differentiation will also not be made between classical chromatographic systems and HPLC since, in essence, it is only the bead or particle size which leads to the higher performance (e.g. throughput, resolution) in the latter method.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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