Introduction

Dyes employed in protein and enzyme purification are synthetic hydrophilic molecules bearing a reactive, usually a chlorotriazine, moiety by which they can easily be attached to various polymeric supports. Among the various known reactive dye ligands, Cibacron Blue 3GA or F3GA (CB3GA, Figure 1A), an ortho-isomer of CI Reactive Blue 2, has attracted most attention from biotechnologists in protein purification. The foundations of the important role of CB3GA may well be attributed to pure historical accident; an anomalous gel permeation chromatogra-phy run of pyruvate kinase when using blue dextran as a void volume marker. It was later found that the blue chromophore, CB3GA, was responsible for binding the enzyme thus leading to co-elution of enzyme and blue dextran. As with many critical but unexpected discoveries, the importance and breadth of applications were hardly appreciated in those early days. Since then, CB3GA and other triazinyl dyes have been immobilized on to various supports and used in the affinity purification of many proteins and enzymes.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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