Molecular imprinting is now recognized as one of the most rapid and powerful methods for creating tailor-made synthetic receptors with strong, yet selective, affinities for a diverse selection of analytes. The imprinting of small organic compounds, metal ions and peptides is well developed and almost routine, and the imprinting of much larger analytes, such as proteins and cells, has also now been demonstrated. The impressive molecular recognition characteristics of molecularly imprinted materials, allied to their highly robust physical nature, makes them ideally suited for numerous applications in affinity separation. This article will outline the general principles behind molecular imprinting and the generic approaches to the preparation of imprinted materials. Particular emphasis will be placed on their role as affinity materials in separation science.

affinity purification. Journal of Chromatography B 715: 147-152.

Kang K, Ryu D, Drohan WN and Orthner CL (1992) Effect of matrices on affinity purification of protein C. Biotechnology and Bioengineering 39: 1086-1096.

Kaster JA, de Oliveira W, Glasser WG and Velander WH (1993) Optimization of pressure-flow limits, strength, interparticle transport and dynamic capacity by hydrogel solids content and bead size in cellulose immunosorbents. Journal of Chromatography 648: 79-90.

Orthner CL, Highsmith FA, Tharakan J et al. (1991) Comparison of the performance of immunosorbents prepared by site-directed or random coupling of monoclonal antibodies. Journal of Chromatography 558: 55-70.

Phillips TM (1989) High-performance immunoaffinity chromatography. Advances in Chromatography 29: 133-173.

Ubrich N, Rivat C, Vigneron C and Maincent P (1998) Microporous particles designed as stable immuno-sorbents. Biotechnology and Bioengineering 58: 581-586.

Velander WH, Subramanian A, Madurawe RD and Orthner CL (1992) The use of Fab-masking antigens to enhance the activity of immobilised antibodies. Biotechnology and Bioengineering 39: 1013-1023.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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