Bile acids, the major components of the bile fluid, play a significant role in lipid metabolism. After formation in the liver and storage in the gall bladder, the bile is secreted in the duodenum. Due to their faecal bile acids could be quantitatively derivatized and eluted from the faeces and bile acids could be quantitated in the presence of other faecal components. The method is also applicable to plasma bile acid measurement. Figure 2 shows plasma and faecal bile acids in a healthy volunteer determined as the n-butyl ester-trimethylsilyl ether derivatives. Since the extra steps of extraction of bile acids and their purification are eliminated, the method can easily be adapted for routine screening of samples for bile acid analysis.

See also: Il/Chromatography: Gas: Delectors: Mass Spectometry; Derivatization; Solid Phase Extraction. Ill/Acids: Gas Chromatography; Liquid Chromatography; Thin-layer (Planar) Chromatography. Extraction: Supercritical Fluid Extraction.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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