Immobilized Metal Ion Chromatography

Table 2 Abbreviations, names and functional structures of chelators

Name

Full name

Dentation

Structure

IDA

Iminodiacetic acid

3

&N(CH2COOH)2

TACN

1,4,7-Triazocyclonanane

1 1

NTA

Nitrilotriacetic acid

4

& CH(COOH)N(CH2COOH)2

TREN

Tris(2-aminoethyl)amine

4

& NHCH2CH2N(CH2CH2NH2)2

Talon

Proprietary

4

Proprietary

TED

Tris(carboxymethly)ethylenediamine

5

& N(CH2COOH)CH2CH2N

(CH2COOH)2

& Indicates chosen form of linkage to a chromatographic support, usually with a suitable spacer. - Indicates atoms involved in metal ion coordination.

& Indicates chosen form of linkage to a chromatographic support, usually with a suitable spacer. - Indicates atoms involved in metal ion coordination.

Whether purifying proteins using native exposed his-tidines, post-translation modifications with phosphate or via an engineered 6His tag, IMAC provides a versatile and relatively gentle method with the potential to provide greater than 90% purity in a single chromatographic step.

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