Horizontal Electrophoresis

Before electrophoresis, the gel is taken out of the cassette. A few drops of kerosene are put on the cooling plate of the opened electrophoretic apparatus (Figure 7) and the gel, firmly adhering to the polyester foil, is placed on it, carefully avoiding the inclusion of air bubbles. Both ends of the gel are connected with the buffer vessels by paper wicks or a household sponge-like material. A 15-30 min pre-electrophor-

esis is performed at 1000 V (50Vcm_1). Then the slots are filled with protein solution (or electrode buffer) and the power is turned on again at a voltage of 1000 V for approximately 2 h. Afterwards the gel, fixed on the polyester foil, may be stained for proteins or (iso)enzymes (Figure 8).

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment