High Performance Affinity Chromatography

When the support is made of noncompressible, spherical particles of small diameter (e.g. 5-20 |im) and narrow size distribution (e.g. 0.2-2.0 |im) the technique is termed high performance affinity chromatography (HPAC). Silica-based supports have been used with dyes for HPAC, although silica has serious drawbacks: poor chemical stability in an alkaline environment; the need for derivatization with organofunctional silanes prior to dye immobilization; and relatively small pore size. In spite of its problems, silica is still used in HPAC mainly on an analytical scale. Synthetic high performance polyhydroxylic particles probably offer a better alternative to silica for HPAC. The chemistry of support activation and dye immobilization is generally the same as that for softer materials, as described earlier.

A different concept for column packings was introduced by the following two materials: deformed nonporous high performance agarose; and nonporous fibres. The former support consists of 12-15 |im diameter nonporous beads which, when compressed, are claimed to result in superior resolution at high flow rates. The latter support consists of nonporous fibre-form quartz material of mean diameter 0.5 | m. This material has first to be silylated prior to proceeding with ligand immobilization. The above materials are potentially suitable for use with dye ligands.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

Get My Free Ebook

Post a comment