Heterobifunctional Ligand Affinity Chromatography

The principle of heterobifunctional affinity ligand chromatography requires that a soluble heterobifunctional affinity ligand possessing two different affinity moieties is available. This alone may be a drawback for the technique, when considering factors such as labour and cost. One moiety is destined to interact in solution with the targeted protein for purification, while the second moiety should be able to recognize and bind to a suitable affinity adsorbent. A model system based on this principle has been developed for purifying lactate dehydrogenase (LDH). In this case, the soluble het-erobifunctional ligand consisted of the dye CB3GA chemically linked to soya bean trypsin inhibitor, whereas immobilized trypsin is used in an affinity column for binding the soluble complex trypsin inhibitor • CB3GA-LDH.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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