Future Perspectives

The isolation and separation of biologically active compounds and xenobiotics using magnetic affinity techniques are a relatively new approach and still under development. Due to the commercial availability of magnetic affinity particles and kits these techniques are currently used mainly in molecular biology (especially for separation of nucleic acids) and as parts of the kits for the determination of selected analytes using magnetic ELISA and related techniques (especially determination of clinical markers and environmental contaminants). Up to now small-scale separations prevail and thus the full potential of these techniques has not been fully exploited.

It can be expected that further development will be focused on two areas. The first one will be oriented to the laboratory-scale application of magnetic affinity separation techniques in biochemistry and related areas (isolation of a variety of both low- and high-molecular weight substances of various origins directly from crude samples thus reducing the number of purification steps) and in biochemical and environmental analysis (application of immunomagnetic particles for separation of target analytes from a mixture followed by their detection using ELISA and related principles). Such a type of analysis enables portable assay systems to be constructed for the detection and determination of environmental contaminants directly on site or for near-patient analysis of various disease markers. Alternatively, fully automated systems for the detection of clinical markers will be constructed.

In the second area, larger-scale (industrial) systems will be developed and used for the isolation of biologically active compounds directly from the crude culture media, wastes from food industry, etc. It is not expected that large amounts of low-cost products will be isolated using magnetic techniques, but attention will be directed to the isolation of minor, but highly valuable components present in raw materials. Of course, prices of magnetic carriers have to be lowered, and special types of low-cost, biotechnology-applicable magnetic carriers prepared by simple and cheap procedures have to become available. Magnetic separations could thus be the technique for the 21st century.

See Colour Plates 61, 62.

See also: I/Affinity Separation. II/Affinity Separation: Immunoaffinity Chromatography. Extraction: SolidPhase Extraction; Solvent Based Separation. III/Catalyst Studies: Chromatography: Isolation Magnetic Techniques; DNA. Immunoaffinity Extraction: RNA. Appendix 1/Essential Guides for Isolation/Purification of Cells: Essential Guides for Isolation/Purification of Enzymes and Proteins: Essential Guides for Isolation/Purification of Nucleic Acids. Appendix 2/Essential Guides to Method Development in Affinity Chromatography.

Solar Panel Basics

Solar Panel Basics

Global warming is a huge problem which will significantly affect every country in the world. Many people all over the world are trying to do whatever they can to help combat the effects of global warming. One of the ways that people can fight global warming is to reduce their dependence on non-renewable energy sources like oil and petroleum based products.

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